Angiotensin converting enzyme 2 (ACE2) is the cellular entry receptor for many SARS-like coronaviruses. SARS-CoV and SARS-CoV-2 bind and enter human cells via the ACE2 receptor. This mouse line expresses human ACE2 under the control of HFH4, a lung ciliated epithelial promoter. HFH4-hACE2-expressing mice are sensitized to infection by SARS-like coronaviruses when compared with wild-type mice. Wild-type mice do not develop clinical disease, however, this mouse strain was developed to express human ACE2 which allows for testing in mice that develop clinical disease upon infection. When infected with SARS-CoV Urbani, HFH4-hACE2-expressing mice experienced no weight loss for the first 3 days, but then experienced rapid weight loss and death between days 4 and 5. This mouse line shows value as a model to study coronavirus pathogenesis and to test therapeutic and vaccine efficacy against such coronaviruses. Related Publications:
This research tool is available from the Mutant Mouse Resource and Research Center (MMRRC) at the following link: https://www.mmrrc.org/catalog/sds.php?mmrrcid=66719.