An improved sensor has been developed for various analytes, including nitric oxide. Electrochemical techniques for in situ sensing currently offer the highest spatial and temporal resolution, but unmodified electrodes suffer from a lack of selectivity and performance degradation in complex biological media due to interferents and biofouling. The present technology improves the performance of the sensor electrode by coating it with a composite membrane comprised of a selectively permeable electropolymerized layer and a xerogel matrix known to prolong performance. Use of both components in one system demonstrated continuous, selective detection of nitric oxide in proteinaceous media over the course of 24 hours.
· Improved selectivity and stability compared to unmodified or single membrane-coated electrodes
· Continuous monitoring of NO release from cultured inflammatory cells
· Composite membrane amenable for use on electrodes of all sizes and geometries